sox 9  (OriGene)

Journal: Cell Reports Medicine

Article Title: Denosumab stimulates spermatogenesis in infertile men with preserved Sertoli cell capacity

doi: 10.1016/j.xcrm.2024.101783

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-SOX9 , Millipore, Germany , Cat#AB5535; RRID: AB_2239761.

Techniques: Polymer, Plasmid Preparation, Recombinant, Ex Vivo, Labeling, Enzyme-linked Immunosorbent Assay, Software, Microscopy, Cytometry, Fluorescence, FACS, Flow Cytometry

Journal: Cells

Article Title: The Novel-m0230-3p miRNA Modulates the CSF1/CSF1R/Ras Pathway to Regulate the Cell Tight Junctions and Blood–Testis Barrier in Yak

doi: 10.3390/cells13151304

Figure Lengend Snippet: Targeting relationship between target gene CSF1 and novel-m0230-3p. ( A ) IF staining identified isolated yak SCs using antibodies against SOX9 (green) and β-tubulin (red); magnification, 20×. ( B ) Immunofluorescence staining identified isolated yak SCs using antibodies against WT1 (green) and β-tubulin (red); magnification, 20×. ( C ) Binding site of CSF1 and novel-m0230-3p. ( D ) Luciferase activity in SCs after co-transfection with mimics of novel-m0230-3p (100 nM) or mimic NC (100 nM) and pmirGLO-CSF1 3′-UTR-WT (400 ng) or pmirGLO-CSF1 3′-UTR-MUT (400 ng). Values represent mean ± SD; n = 3, ** p < 0.01.

Article Snippet: The cells were incubated overnight at 4 °C with rabbit anti-MCSF (1:80, T55362, Abmart, Shanghai, China), rabbit anti-WT1 (1:100, 12609-1-AP, Proteintech, Wuhan, China), rabbit anti-SOX9 (1:100, bs-10725R, Bioss, Beijing, China), or rabbit anti-CSF1R (1:100, TP70252, Abmart), while the sections were incubated overnight at 4 °C with rabbit anti-β-catenin (1:100, 51067-2-AP, Proteintech) or rabbit anti-ZO1 (1:100, 21773-1-AP, Proteintech).

Techniques: Staining, Isolation, Immunofluorescence, Binding Assay, Luciferase, Activity Assay, Cotransfection